منابع مشابه
Genetics of Somatic Mammalian Cells
A convenient, reliable method for chromosome delineation of animal cells grown as monolayers on glass has been applied to human, opossum, and Chinese hamster cells. Tissue cultured cells from 5 different, normal organs of 7 different human subjects uniformly displayed the expected chromosome number of 46 and showed no variations in morphology or number other than the expected sex differences an...
متن کاملGenetics of somatic mammalian cells: biochemical genetics of Chinese hamster cell mutants with deviant purine metabolism.
Studies are presented on the biochemical genetics of 30 adenine-requiring mutants of the Chinese hamster ovary cell which were induced by mutagenesis and selected by the BrdU-visible light technique. Representative experiments conducted with these mutants include: hybridization with each other; hybridization with normal human cells; nutritional analysis; biochemical analysis with radioactively ...
متن کاملReprogramming mammalian somatic cells.
Somatic cell nuclear transfer (SCNT), the technique commonly known as cloning, permits transformation of a somatic cell into an undifferentiated zygote with the potential to develop into a newborn animal (i.e., a clone). In somatic cells, chromatin is programmed to repress most genes and express some, depending on the tissue. It is evident that the enucleated oocyte provides the environment in ...
متن کاملGenetics of Somatic Mammalian Cells* Hi. Long-term Cultivation of Euploid Cells from Human
The animal cell lines most readily cultivated by current tissue culture techniques are usually aneuploid in chromosomal constitution and often have originated in malignant tissue (1). Most workers report that normal cells from adult individuals either fail to grow in vitro, or grow only for brief periods, usually a few weeks or months, after which proliferation ceases (2). Application of recent...
متن کاملGenetics of somatic mammalian cells: lethal antigens as genetic markers for study of human linkage groups.
The antigen that causes killing of at least 98% of a human cell population treated with a 1% solution of a specific rabbit antiserum in the presence of complement is a sensitive genetic marker. The rapid loss of human chromosomes in human-Chinese hamster cell hybrids makes possible a convenient test of linkage relationships with this marker. Hybrid clones with and without the lethal antigen wer...
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ژورنال
عنوان ژورنال: Journal of Experimental Medicine
سال: 1958
ISSN: 1540-9538,0022-1007
DOI: 10.1084/jem.108.6.945